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SP4 Antibody

CAT: 0800-R30964Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30964Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Transcription factor Sp4, also known as SPR-1, and HF1B, is a protein that in humans is encoded by the SP4 gene. It is a multigene family of SP proteins. Northern blot analysis shows that it is abundantly expressed as a 7.5-kb message in brain, but barely detectable in other human cell lines. It is an activator protein like SP1. However, in contrast to SP1, Sp4 is not able to act synergistically through adjacent binding sites.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
Q02446
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the N-terminus of human Transcription factor Sp4 (ENNNKKPKTSGSQDSQ) was used as the immunogen for this SP4 antibody (100% rat homology) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Sp4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SP4 antibody is available for research use only.
Storage Conditions
After reconstitution, the SP4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Transcription factor SP4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Western blot testing of SP4 antibody and Lane 1: rat spleen; and human samples 2: SGC; 3: HeLa; 4: Jurkat; 5: A549 cell lysate. Predicted/observed molecular weight ~82kDa.

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