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Splicing factor 1 Antibody / SF1

CAT: 0800-R30963Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30963Size:100 µg
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Description
Splicing factor 1, also known a sSF1, Zinc finger protein 162 (ZNF162), ZFM162, Transcription factor ZFM1, and Mammalian branch point-binding protein (BBP or mBBP), is a protein that in humans is encoded by the SF1 gene. It ispredicted 623-amino acid protein. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary. All of the isoforms contain a KH domain, a sequence motif present in proteins playinig a major role in regulating cellular RNA metabolism.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q15637
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the N-terminus of human Splicing factor 1 (DFPSKKRKRSRWNQDTMEQK) was used as the immunogen for this Splicing factor 1 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Splicing factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Splicing factor 1 antibody is available for research use only.
Storage Conditions
After reconstitution, the Splicing factor 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Splicing factor 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
IHC staining of FFPE rat small intestine tissue with Splicing factor 1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

UniProtKB · Q15637

Splicing factor 1

SF01_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q15637
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SF1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B7Z1Q1, C9JJE2, Q14818, Q14819, Q15913, Q8IY00, Q92744, Q92745, Q969H7, Q9BW01, Q9UEI0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationMethylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Ligand

Metal-bindingZinc

Biological process

mRNA processingmRNA splicingTranscriptionTranscription regulation

Cellular component

NucleusSpliceosome

Molecular function

RepressorRNA-binding

Domain

Zinc-finger

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