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Orai1 Antibody

CAT: 0800-R30956Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30956Size:100 µg
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Description
Orai calcium release-activated calcium modulator 1, also known as CRACM1, and TMEM142A, is a calcium selectiveion channel that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function/mutation of Orai1 causes Severe combined immunodeficiency (SCID) in humans. The mammalian Orai family has two additional homologs, Orai2 and Orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers. Prakriya et al. (2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of two conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion. They showed that Orai1 is a PM protein, and that CRAC channel function is sensitive to mutation of two conserved acidic residues in the transmembrane segments.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
Q96D31
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human Orai1 (EFARLQDQLDHRGDHPLTPGSHYA) was used as the immunogen for this Orai1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Orai1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Orai1 antibody is available for research use only.
Storage Conditions
After reconstitution, the Orai1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Orai1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Orai1 antibody and SKOV lysate; Predicted molecular weight ~ 33 kDa; Observed size 45~50 kDa due to glycosylation

UniProtKB · Q96D31

Calcium release-activated calcium channel protein 1

ORAI1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q96D31
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ORAI1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q3MHV3, Q6DHX2, Q96BP7, Q96K71
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

Adaptive immunityCalcium transportImmunityIon transportTransport

Coding sequence diversity

Alternative initiation

Ligand

Calcium

Molecular function

Calcium channelCalmodulin-bindingIon channel

Cellular component

Cell membraneMembrane

Disease

Disease variant

PTM

GlycoproteinPhosphoproteinUbl conjugation

Domain

TransmembraneTransmembrane helix

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