LASP1 Antibody

• Description:

  LIM and SH3 domain protein 1 is a protein that in humans is encoded by the LASP1 gene. This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization. LASP1 has been shown to interact with Zyxin. Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8% of primary breast cancers. In most of these cancers, LASP1 and ERBB2 were simultaneously overexpressed.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

• UniProt:

  Q14847

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  An amino acid sequence from the C-terminus of human LASP1 (YRRPLEQQQPHHIPTSA) was used as the immunogen for this LASP1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the LASP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This LASP1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the LASP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the LASP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of LASP1 antibody and Lane 1: HeLa; 2: MCF-7 cell lysate. Predicted molecular weight: 30kDa.