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GRP75 Antibody / HSPA9

CAT: 0800-R30886Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30886Size:100 µg
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Description
Heat shock 70kDa protein 9, also known as GRP75, mot-2, mthsp75, PBP74, HSPA9B, and Mortalin, is a highly conserved member of the HSP70 family of proteins. It functions as a chaperone in the mitochondria, cytoplasm, and centrosome. This protein plays a role in the control of cell proliferation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
P38646
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human GRP75 (DTETKMEEFKDQLPADECN) was used as the immunogen for this GRP75 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Grp75 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This GRP75 antibody is available for research use only.
Storage Conditions
After reconstitution, the GRP75 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the GRP75 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of 1) human HeLa, 2) human A549, 3) human Jurkat, 4) human HepG2, 5) rat testis, 6) mouse testis and 7) mouse NIH 3T3 cell lysate with GRP75 antibody. Expected molecular weight: 70~75 kDa.

UniProtKB · P38646

Stress-70 protein, mitochondrial

HSPA9_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P38646
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HSPA9
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
mt-HSP70
EC number
3.6.4.10
Processing
Precursor
Secondary accessions
B2RCM1, P30036, P31932, Q1HB43, Q53H23, Q6GU03, Q9BWB7, Q9UC56
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationMethylationPhosphoprotein

Ligand

ATP-bindingNucleotide-binding

Molecular function

ChaperoneHydrolase

Cellular component

CytoplasmMitochondrionNucleus

Disease

Disease variantDwarfism

Domain

Transit peptide
Product Datasheet
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