Products for Research Use Only
RegisterLogin

Vinculin Antibody

CAT: 0800-R30874Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-R30874Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
In mammalian cells, Vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a 3-generation family, Mulligan et al. (1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to D10S22. It is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is though to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively-spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. The human protein exhibits a greater-than-95% sequence identity to the chicken protein. Vinculin is a 117-kDa cytoskeletal protein with 1066 amino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. It has a globular head domain that contains binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin, and lipids.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
P18206
Host
Rabbit
Reactivity
Mouse
Immunogen
Amino acids 173-188 (KMIDERQQELTHQEHR) were used as the immunogen for this Vinculin antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Vinculin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Vinculin antibody is available for research use only.
Storage Conditions
After reconstitution, the Vinculin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic and cell membrane
Image Legend
Western blot testing of Vinculin antibody and Lane 1: rat heart; 2: (r) brain; 3: (r) liver; 4: human U87; 5: (h) SMMC-7721; 6: mouse HEPA; 7: (h) HeLa; 8: (h) HT1080 cell lysate. Predicted molecular weight ~124 kDa.

Related Products

CatalogName
F54927-0.08MLVCL Antibody / Vinculin
F54927-0.4MLVCL Antibody / Vinculin
RQ4624NONO Antibody / p54nrb Antibody
F53954-0.2MLAntibody
F53954-0.05MLAntibody
F53952-0.2MLAntibody
F53952-0.05MLAntibody
V8674-20UGInhibin alpha Antibody / INHA antibody
V8674SAF-100UGInhibin alpha Antibody / INHA antibody
V8674-100UGInhibin alpha Antibody / INHA antibody

Alternative Products

CatalogName
10R-V105aVinculin antibody

Popular Products