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NDUFA1 Antibody

CAT: 0800-R30746Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30746Size:100 µg
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Description
NADH-ubiquinone oxidoreductase 1 alpha subcomplex 1 is a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity. The findings established that the protein is absolutely essential for an active complex I in mammals. The protein is one of about 31 components of the hydrophobic protein (HP) fraction of complex I which is involved in proton translocation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunohistochemistry (Frozen) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
O15239
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human NDUFA1 (ISGVDRYYVSKGLENID) was used as the immunogen for this NDUFA1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the NDUFA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NDUFA1 antibody is available for research use only.
Storage Conditions
After reconstitution, the NDUFA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the NDUFA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic (mitochondria)
Image Legend
IHC staining of FFPE human skeletal muscle tissue with NDUFA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · O15239

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

NDUA1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O15239
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NDUFA1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Disease

Disease variantPrimary mitochondrial disease

Biological process

Electron transportRespiratory chainTransport

Cellular component

MembraneMitochondrionMitochondrion inner membrane

Domain

TransmembraneTransmembrane helix

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