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Cytoglobin Antibody

CAT: 0800-R30742Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30742Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cytoglobin (CYGB), also called HGB or STAP, is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. The CYGB gene contains four exons and spans about 9 kb. Cytoglobin has many elements common to vertebrate globins, including invariant histidine residues, and the amino acids that form the heme pocket share similarity with pentacoordinate myoglobin. In contrast to the high oxygen affinities displayed by most hexacoordinate hemoglobins, the characteristics of CYGB indicate that it can facilitate oxygen transport. Because the oxygen affinity of CYGB is more similar to myoglobin than to neuroglobin, and the oxy form of CYGB resists autooxidation, CYGB is proposed to represent a tissue oxygen reservoir.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
Q8WWM9
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the N-terminus of human Cytoglobin (FVNFPSAKQYFSQFKHMED) was used as the immunogen for this Cytoglobin antibody (100% rat homology) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Cytoglobin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Cytoglobin antibody is available for research use only.
Storage Conditions
After reconstitution, the Cytoglobin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Cytoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Cytoglobin antibody and Lane 1: rat brain; 2: (r) small intestine; 3: (r) liver; 4: (r) kidney; 5: human SGC; 6: (h) COLO320; 7: SMMC-7721; 8: PANC; 9: HeLa cell lysate. Predicted/observed molecular weight: ~21 kDa.

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