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IL5RA Antibody

CAT: 0800-R30700Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30700Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interleukin 5 receptor alpha, also known as CD125, is a subunit of the Interleukin-5 receptor. The protein encoded by the IL5RA gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor which is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5) . The IL5RA gene is mapped to 3p26-p24 by Southern blot analysis of DNA from a panel of mouse-human hybrid somatic cell lines complemented by in situ hybridization. And its cytogenetic location is 3p26.2. Within a 15-residue stretch of IL5RA, the C-terminal phenylalanine is critical. The binding of this protein to IL5 depends on the beta subunit which is activated by the ligand binding and is required for the biological activities of IL5.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
Q01344
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the N-terminus of human IL5RA (NPDQEQRNVNLEYQVK) was used as the immunogen for this IL5RA antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the IL5RA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This IL5RA antibody is available for research use only.
Storage Conditions
After reconstitution, the IL5RA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the IL5RA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of IL5RA antibody and Lane 1: A549; 2: SMMC-7721; 3: U87; 4: 293T; 5: PANC; 6: HeLa; 7: Jurkat; 8: Raji; 9: CEM cell lysate. The 420 amino acid protein is routinely visualized between 46~60KD, depending on level of glycosylation.

UniProtKB · Q01344

Interleukin-5 receptor subunit alpha

IL5RA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q01344
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL5RA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA
EC number
—
Processing
Precursor
Secondary accessions
B3IU77, B4E2G0, Q14633, Q15469, Q6ISX9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoprotein

Cellular component

Membrane

Molecular function

Receptor

Domain

RepeatSignalTransmembraneTransmembrane helix

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