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HSP105 Antibody

CAT: 0800-R30688Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30688Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Heat shock protein 105, also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105alpha and HSP105beta are upregulated in HeLa cells exposed to heat shock. HSP105alpha, but not HSP105beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Additionally, it may constitute a major determinant for both prognosis and treatment response in colorectal cancer.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL
UniProt
Q92598
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 713-733 (EVMEWMNNVMNAQAKKSLDQD-human) were used as the immunogen for this HSP105 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Hsp105 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HSP105 antibody is available for research use only.
Storage Conditions
After reconstitution, the Hsp105 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the HSP105 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of HSP105 antibody and Lane 1: rat ovary ; 2: A549 cell lysate; 3: U87 cell lysate; 4: HeLa cell lysate. Expected molecular weight: 105-110 kDa.

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