ATF4 Antibody

• Description:

  Activating Transcription Factor 4, also know as CREB2, belongs to the large ATF/CREB family of transcription factors which bind DNA via their basic region and dimerize via their leucine zipper domain to form a variety of homo- and heterodimers to regulate gene transcription. Members of this family share significant sequence similarity within a leucine zipper DNA-binding motif and an adjacent basic region. Unlike CREB, which activates transcription from CRE-containing promoters, CREB2/ATF4 functions as a specific repressor of CRE-dependent transcription. The transcriptional repressor activity resides within the C-terminal leucine zipper and basic domain region of the ATF4 protein.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P18848

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids 332-351 (QYLKDLIEEVRKARGKKRVP) were used as the immunogen for this ATF4 antibody (100% homologous in human, mouse and rat) .

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the ATF4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This ATF4 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the ATF4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the ATF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of ATF4 antibody and Lane 1: A431; 2: Raji; 3: CEM; 4: HUT cell lysate. Predicted molecular weight ~39kDa.