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Apelin Antibody

CAT: 0800-R30574Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30574Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei that acts on specific receptors located on vasopressinergic neurons. The gene is at Xq25-q26.3, based on sequence similarity between the Apelin sequence and a chromosome X PAC clone. This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
Q9ULZ1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of Apelin (RKFRRQRPRLSHKGPMPF) was used as the immunogen for this Apelin antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Apelin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Apelin antibody is available for research use only.
Storage Conditions
After reconstitution, the Apelin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Apelin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Apelin antibody and Lane 1: U87; 2: MCF-7; 3: HeLa; 4: MM453 cell lysate

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