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EGF Antibody

CAT: 0800-R30469Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30469Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a mature 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
P01132
Host
Rabbit
Reactivity
Mouse
Immunogen
An amino acid sequence from the C-terminus of mouse EGF (YSGDRCQTRDLRWWELR) was used as the immunogen for this EGF antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the EGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This EGF antibody is available for research use only.
Storage Conditions
After reconstitution, the EGF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the EGF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of EGF antibody and Lane 1: recombinant mouse partial protein 10ng; 2: 5ng; 3: 2.5ng
Product Datasheet
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