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RANK Antibody

CAT: 0800-R30453Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30453Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Receptor Activator of Nuclear Factor K B, also known as TRANCE Receptor and Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A), is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is also expressed on dendritic cells and facilitates immune signaling. RANKL (Receptor Activator for Nuclear Factor K B Ligand) is found on the surface of stromal cells, osteoblasts, and T cells. By analysis of somatic cell and radiation hybrid panels, Anderson et al. (1997) mapped the RANK gene to 18q22.1.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
Q9Y6Q6
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the N-terminus of human RANK (QIAPPCTSEKHYEHLG) was used as the immunogen for this RANK antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the RANK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RANK antibody is available for research use only.
Storage Conditions
After reconstitution, the RANK antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the RANK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membranous
Image Legend
Western blot testing of RANK antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng

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