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IL-10 Antibody

CAT: 0800-R30421Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30421Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interleukin-10, also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse il10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P22301
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the N-terminus of human Interleukin-10 (DAFSRVKTFFQMKDQ) was used as the immunogen for this IL-10 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the IL-10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This IL-10 antibody is available for research use only.
Storage Conditions
After reconstitution, the IL-10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the IL-10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of IL-10 antibody and Lane 1: Recombinant human IL-10 protein 10ng; 2: 5ng; 3: 2.5ng

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