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Podocin Antibody

CAT: 0800-R30383Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30383Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Podocin (PDCN), also called NPHS2, is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. PDCN is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL
UniProt
Q9NP85
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 368-383 (KPVEPLNPKKKDSPML) were used as the immunogen for this Podocin antibody (100% homologous human and mouse) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
The lyophilized Podocin antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Podocin antibody is available for research use only.
Storage Conditions
The lyophilized Podocin antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Titration of the Podocin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Podocin antibody and rat kidney tissue lysate. Predicted molecular weight ~42 kDa.

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