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Phospholamban Antibody

CAT: 0800-R30366Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30366Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca (2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells. McTiernan et al. (1999) observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles, while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217 bp upstream of the transcription start site.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P26678
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
N-terminus amino acids EKVQYLTRSAIRRAS were used as the immunogen for this Phospholamban antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Phospholamban antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Phospholamban antibody is available for research use only.
Storage Conditions
After reconstitution, the Phospholamban antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Phospholamban antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) rat heart, 2) rat heart, 3) mouse heart and 4) mouse heart tissue lysate with Phospholamban antibody. Predicted molecular weight: 6/12/18/24 kDa (monomer/dimer/oligomers) .

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