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NSE Antibody

CAT: 0800-R30242Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30242Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NSE (Neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
P09104
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human NSE (EARFAGHNFRNPSVL) was used as the immunogen for this NSE antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the NSE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NSE antibody is available for research use only.
Storage Conditions
After reconstitution, the NSE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the NSE antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membranous
Image Legend
Western blot testing of NSE antibody and rat brain tissue lysate. Predicted molecular weight: ~47 kDa.

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