BDNF Antibody

• Description:

  Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P23560

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An amino acid sequence from the N-terminal of human BDNF (HSDPARRGELSVCDSISEWV) was used as the immunogen for this BDNF antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the BDNF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This BDNF antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the BDNF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the BDNF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of BDNF antibody and Lane 1: rat brain; 2: mouse brain; 3: MCF-7; Predicted size: 28KD; Observed size: 37KD