FE65 Antibody

• Description:

  APBB1 is also known as RIR or FE65. The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimers disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimers disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

• Specifications:

  Western blot: 0.5-1 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Human partial recombinant protein (AA 295-613) was used as the immunogen for this FE65 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the FE65 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This FE65 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the FE65 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the FE65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, membrane

• Image Legend:

  Western blot testing of FE65 antibody and mouse brain lysate. Predicted molecular weight: ~77kDa; the 97kDa isoform can be cleaved to yield a 65kDa C-terminal form.