IL3 Antibody

CAT: 0800-R30148Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R30148Size:100 µg
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Description
Interleukin 3 is a glycoprotein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (rat protein tested) ; request BSA free format for coating
Host
Rabbit
Reactivity
Rat
Immunogen
Rat partial recombinant protein (AA 28-166) was used as the immunogen for this IL3 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA (protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the IL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This IL3 antibody is available for research use only.
Storage Conditions
After reconstitution, the IL3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the IL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of IL3 antibody and Lane 1: PC-12; 2: NRK; 3: RH35; The glycoprotein is routinely visualized between 18~35 kDa.

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