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Vinculin Antibody

CAT: 0800-R30091Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30091Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and the two proteins exhibit a high level of sequence identity (greater than 95%) .
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, IHC (Frozen) : 2-4 µg/mL for formalin or acetone fixed tissues, ICC/IF: 2-4 µg/mL
Host
Mouse
Reactivity
Human, Mouse, Rat, Chicken
Immunogen
Human vinculin purified from uterus tissue was used as the immunogen for this Vinculin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
HVIN-1
Applications
WB, IHC-F, ICC/IF
Purity
Unpurified ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the Vinculin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Vinculin antibody is available for research use only.
Storage Conditions
After reconstitution, the Vinculin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of 1) human HeLa, 2) human HepG2, 3) monkey COS-7, 4) human U-87 MG, 5) rat PC-12, 6) rat RH35, 7) mouse HEPA1-6, and 8) mouse NIH3T3 lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
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