Nebulin Antibody

• Description:

  Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.

• Specifications:

  Western blot: 1-2 µg/mL

• Host:

  Mouse

• Reactivity:

  Chicken

• Immunogen:

  Chicken breast nebulin was used as the immunogen for this Nebulin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  NB2

• Applications:

  WB

• Purity:

  Ascites

• Format:

  Ascites

• Buffer:

  0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Reconstitution:

  After reconstitution, the Nebulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Nebulin antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Nebulin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Nebulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4