Myosin Antibody (Skeletal Fast)

• Description:

  Myosin is composed of 2 heavy chains of about 200 kD each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types: the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types. Skeletal Myosin (fast), also known as MYL1, mapped to the region 2q32.1-qter.

• Specifications:

  Western blot: 1-2 µg/mL, IHC (FFPE) : 1-2 µg/mL

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, Rabbit

• Immunogen:

  Rabbit muscle myosin was used as the immunogen for this Myosin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  MY-32

• Applications:

  WB, IHC-P

• Purity:

  Ascites

• Format:

  Ascites

• Buffer:

  0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Reconstitution:

  After reconstitution, the Myosin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Myosin antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Myosin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Myosin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of Myosin antibody and rat skeletal muscle tissue lysate