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HSP70 Antibody

CAT: 0800-R30043Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30043Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Heat shock protein 70 isolated from bovine brain was used as the immunogen for this HSP70 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
BRM-22
Applications
WB, IHC-P
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the HSP70 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HSP70 antibody is available for research use only.
Storage Conditions
After reconstitution, the HSP70 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting points. Titration of the HSP70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC staining of FFPE human breast cancer tissue with HSP70 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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