GFAP Antibody

• Description :

  Glial fibrillary acidic protein is an intermediate filament protein of 52kDa. Its gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in the protein are associated with Alexander disease.

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.4-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL (formalin or acetone fixed tissues), Immunofluorescence: 2-4 µg/mL

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Glial fibrillary acidic protein from pig spinal cord was used as the immunogen for this GFAP antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  G-A-5

• Applications :

  WB, IHC-P, IHC-F, IF

• Purity :

  Unpurified ascites

• Format :

  Ascites

• Buffer :

  0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Reconstitution :

  After reconstitution, the GFAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This GFAP antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the GFAP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the GFAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Immunofluorescent staining of FFPE rat brain tissue with GFAP antibody (green), MBP antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.