GAP43 Antibody

• Description:

  GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IHC (Frozen) : 1-2 µg/mL for formalin or acetone fixed tissues

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  GAP-43 from neonatal rat forebrain membranes was used as the immunogen for this GAP43 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  GAP-7B10

• Applications:

  WB, IHC-P, IHC-F

• Purity:

  Ascites

• Format:

  Ascites

• Buffer:

  0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Reconstitution:

  After reconstitution, the GAP43 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This GAP43 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the GAP43 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the GAP43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, membranous, nuclear

• Image Legend:

  IHC testing of FFPE rat heart tissue with GAP43 antibody. HIER: steamed with pH6 citrate buffer, HRP-staining.