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GAP43 Antibody

CAT: 0800-R30037Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30037Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IHC (Frozen) : 1-2 µg/mL for formalin or acetone fixed tissues
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
GAP-43 from neonatal rat forebrain membranes was used as the immunogen for this GAP43 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
GAP-7B10
Applications
WB, IHC-P, IHC-F
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the GAP43 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This GAP43 antibody is available for research use only.
Storage Conditions
After reconstitution, the GAP43 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the GAP43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membranous, nuclear
Image Legend
IHC testing of FFPE rat heart tissue with GAP43 antibody. HIER: steamed with pH6 citrate buffer, HRP-staining.
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