Desmin Antibody

• Description :

  Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. The DES gene encodes a cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball (1992) found that Desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al. (1989) localized the gene to 2q35. Mutations are responsible for idiopathic dilated cardiomyopathy.

• Specifications :

  Western blot: 2 µg/mL, IHC (FFPE) : 2-4 µg/mL

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat, Rabbit

• Immunogen :

  Desmin from pig stomach was used as the immunogen for this Desmin antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  DE-U-10

• Applications :

  WB, IHC-P

• Purity :

  Unpurified ascites

• Format :

  Ascites

• Buffer :

  0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Reconstitution :

  After reconstitution, the Desmin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This Desmin antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the Desmin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the Desmin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, membranous

• Image Legend :

  IHC-P: Desmin antibody testing of rat heart tissue