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Recombinant Aurora B Antibody

CAT: 0800-R20295-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20295-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant Aurora B antibody reacts to human Aurora kinase B.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1:200-1:500 (1), Western blot: 1:1000-1:2000
UniProt
Q96GD4
Host
Rabbit
Reactivity
Human
Immunogen
A peptide corresponding to the N-terminus of human Aurora kinase B was used as the immunogen for this recombinant Aurora B antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM278
Type
Recombinant
Applications
WB, IHC
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant Aurora B antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant Aurora B antibody is available for research use only.
Storage Conditions
Store the recombinant Aurora B antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant Aurora B antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Location
Nuclear
Image Legend
IHC testing of FFPE human tonsil tissue with recombinant Aurora B antibody at 1:1000.

UniProtKB · Q96GD4

Aurora kinase B

AURKB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q96GD4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
AURKB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.11.1
Processing
—
Secondary accessions
B4DNM4, C7G533, C7G534, C7G535, D3DTR4, J9JID1, O14630, O60446, O95083, Q96DV5, Q9UQ46
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Biological process

Cell cycleCell divisionMitosis

Cellular component

CentromereChromosomeCytoplasmCytoskeletonKinetochoreNucleus

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

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