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Recombinant 5mC Antibody

CAT: 0800-R20245-50UGSize: 50 µgDry Ice: NoHazardous: No
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CAT#:0800-R20245-50UGSize:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
CAS Number
9000-83-3
Specifications
Dot blot: 0.5-2 µg/mL, ELISA: 0.1-1 µg/mL, Immunocytochemistry: 0.5-2 µg/mL, MeDIP: 0.2 µg/mL- 2 µg/mL
Host
Rabbit
Reactivity
Human
Immunogen
BSA-conjugated 5-methylcytosine was used as the immunogen for this recombinant 5mC antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM231
Type
Recombinant
Applications
DB, MeDIP, ICC, ELISA
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant 5mC antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant 5mC antibody is available for research use only.
Storage Conditions
Store the recombinant 5mC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant 5mC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
ELISA of single stranded DNA using recombinant 5mC antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM231 was used as the primary, and an anti-rabbit IgG AKP secondary.
Product Datasheet
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