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Recombinant H3K79ac Antibody

CAT: 0800-R20206-25UGSize: 25 µgDry Ice: NoHazardous: No
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CAT#:0800-R20206-25UGSize:25 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant H3K79ac antibody reacts to Histone H3 acetylated at Lysine 79 (K79ac) . No cross reactivity with other acetylated Lysines in Histone H3.
CAS Number
9000-83-3
Specifications
Western blot: 0.5-2 µg/mL, Immunocytochemistry: 0.5-2 µg/mL, ChIP: 2-10ug/mg of lysate, ELISA: 0.2-1 µg/mL
UniProt
P84243
Host
Rabbit
Reactivity
All Species Expected
Immunogen
An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79ac antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM156
Type
Recombinant
Applications
WB, ELISA, ChIP, ICC
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant H3K79ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant H3K79ac antibody is available for research use only.
Storage Conditions
Store the recombinant H3K79ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant H3K79ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K79ac antibody at 1 ug/ml showed a band of Histone H3 acetylated at Lysine 79 in treated HeLa cells.

UniProtKB · P84243

Histone H3.3

H33_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P84243
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
H3-3A, H3-3B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
P06351, P33155, Q5VV55, Q5VV56, Q66I33, Q9V3W4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationCitrullinationHydroxylationIsopeptide bondLipoproteinMethylationPhosphoproteinUbl conjugation

Cellular component

ChromosomeNucleosome coreNucleus

Disease

Disease variantIntellectual disability

Molecular function

DNA-binding

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