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Recombinant Phosphothreonine Antibody

CAT: 0800-R20193-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R20193-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant Phosphothreonine antibody reacts with threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
CAS Number
9000-83-3
Specifications
Western blot: 1:500 -1:2000 dilution, Immunoprecipitation: 1:100-1:500 dilution, Chromatin IP (ChIP) : 1:100-1:500 dilution, Immunocytochemistry: 1:100-1:500 dilution, Immunohistochemistry: 1:100-1:500 dilution (1)
Host
Rabbit
Reactivity
All Species Expected
Immunogen
Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide was used as the immunogen for this recombinant Phosphothreonine antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM102
Type
Recombinant
Applications
WB, IP, ICC, IHC, ChIP
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant Phosphothreonine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant Phosphothreonine antibody is available for research use only.
Storage Conditions
Store the recombinant Phosphothreonine antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant Phosphothreonine antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Image Legend
ICC of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:500 (PE-secondary, red) and DAPI (blue) .

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