14-3-3 sigma Antibody

• Description:

  14-3-3 proteins are regulators of a diverse group of signaling proteins, including kinases, phosphatases, and transmembrane receptors, and have roles in a wide range of physiological processes including transcription, intracellular signaling, cell cycle control and apoptosis. There are seven 14-3-3 proteins in most mammals: beta, gamma epsilon, zeta, eta, sigma and tau. 14-3-3 sigma, also called Stratifin, is a product of the SFN gene. It is a tumor suppressor gene inactivated via DNA methylation. Reduced or no expression of 14-3-3 sigma is seen in various carcinoma including skin, breast and prostate, suggesting that 14-3-3 gene silencing may be involved in tumor progression.

• Specifications:

  Western blot: 1-3 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A recombinant protein fragment from the internal region of 14-3-3 sigma was used as the immunogen for this antibody. The amino acid sequence is common to the ~30kDa and ~20kDa isoforms.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Aliquot the 14-3-3 sigma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This 14-3-3 sigma antibody is available for research use only.

• Storage Conditions:

  Aliquot the 14-3-3 sigma antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Provided assay concentrations are suggestions only, 14-3-3 sigma antibody titration may be required for optimal results.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human samples with 14-3-3 sigma antibody at 1ug/ml. Predicted molecular weight: ~28kDa.