ANKH Antibody

• Description :

  ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.

• CAS Number :

  9007-83-4

• Specifications :

  Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

• UniProt :

  Q9HCJ1

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  A portion of amino acids 464-492 from the human protein was used as the immunogen for the ANKH antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  FACS, IHC-P, WB

• Purity :

  Antigen affinity purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the ANKH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This ANKH antibody is available for research use only.

• Storage Conditions :

  Aliquot the ANKH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the ANKH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Image Legend :

  Western blot testing of mouse cerebellum tissue lysate with ANKH antibody. Predicted molecular weight ~54 kDa.