Galactosidase alpha Antibody / Gla

• Description:

  GLA is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.

• Specifications:

  Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

• UniProt:

  P06280

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 83-112 from the human protein was used as the immunogen for the Galactosidase alpha antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, IF, IHC-P, WB

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Galactosidase alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Galactosidase alpha antibody is available for research use only.

• Storage Conditions:

  Aliquot the Galactosidase alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Galactosidase alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC testing of FFPE human lung carcinoma tissue with Galactosidase alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.