DNAJA1 Antibody / HDJ2

• Description:

  DnaJ homolog subfamily A member 1 plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. [UniProt]

• Specifications:

  Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

• UniProt:

  P31689

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 294-323 from the human protein was used as the immunogen for the DNAJA1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, IHC-P, WB

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the DNAJA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This DNAJA1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the DNAJA1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the DNAJA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  IHC testing of FFPE human prostate carcinoma tissue with DNAJA1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.