STEA2 Antibody

CAT: 0800-F54829-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
CAT#:0800-F54829-0.08MLSize:0.08 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100
UniProt
Q8NFT2
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 229-258 from the human protein was used as the immunogen for the STEA2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the STEA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This STEA2 antibody is available for research use only.
Storage Conditions
Aliquot the STEA2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the STEA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of human HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide, human HepG2 cell lysate in the 3) absence and 4) presence of immunizing peptide, and mouse spleen tissue lysate in the 5) absence and 6) presence of immunizing peptide with STEA2 antibody. Predicted molecular weight: 48-56 kDa (3 isoforms) .

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