MTHFD1 Antibody / C-1-tetrahydrofolate synthase

• Description:

  MTHFD1 is a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

• Specifications:

  Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:100

• UniProt:

  P11586

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 535-562 from the human protein was used as the immunogen for the MTHFD1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IF, WB, IHC-P

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the MTHFD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This MTHFD1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the MTHFD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the MTHFD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Western blot testing of human Y79 cell lysate with MTHFD1 antibody. Predicted molecular weight ~102 kDa.