UVRAG Antibody

• Description :

  UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI (3) KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.

• CAS Number :

  9007-83-4

• Specifications :

  Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

• UniProt :

  Q9P2Y5

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  A portion of amino acids 666-699 from the human protein was used as the immunogen for the UVRAG antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  IF, IHC-P, WB

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the UVRAG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This UVRAG antibody is available for research use only.

• Storage Conditions :

  Aliquot the UVRAG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the UVRAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Image Legend :

  Immunoprecipitation and western blot analysis using UVRAG antibody with 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1) . Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4) . Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.