EFL1 Antibody / EFTUD1

CAT: 0800-F54540-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
CAT#:0800-F54540-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. [UniProt]
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
UniProt
Q7Z2Z2
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 1062-1090 from the human protein was used as the immunogen for the EFL1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC-P, FACS, WB
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the EFL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This EFL1 antibody is available for research use only.
Storage Conditions
Aliquot the EFL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the EFL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC testing of FFPE human skeletal muscle tissue with EFL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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