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SMAD1 Antibody

CAT: 0800-F54496-0.2MLSize: 0.2 mLDry Ice: NoHazardous: No
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CAT#:0800-F54496-0.2MLSize:0.2 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) . SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP) -mediated cardiac-specific gene expression.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25
UniProt
Q15797
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the SMAD1 antibody.
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
1356CT119.18.55
Applications
WB, IHC-P, IF
Purity
Protein G affinity
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SMAD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SMAD1 antibody is available for research use only.
Storage Conditions
Aliquot the SMAD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear, cytoplasmic
Image Legend
IHC testing of FFPE human colon tissue with SMAD1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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