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PCNA Antibody

CAT: 0800-F54492-0.05MLSize: 0.05 mLDry Ice: NoHazardous: No
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CAT#:0800-F54492-0.05MLSize:0.05 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25
UniProt
P12004
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the PCNA antibody.
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
1655CT506.10.26
Applications
WB, FACS, IHC-P
Purity
Protein G affinity
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PCNA antibody is available for research use only.
Storage Conditions
Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
IHC testing of FFPE human ovarian cancer tissue with PCNA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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