Products for Research Use Only
RegisterLogin

IRF3 Antibody

CAT: 0800-F54481-0.05MLSize: 0.05 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-F54481-0.05MLSize:0.05 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Key transcriptional regulator of type I interferon (IFN) -dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25
UniProt
Q14653
Host
Mouse
Reactivity
Human, Monkey
Immunogen
Recombinant human protein was used as the immunogen for the IRF3 antibody.
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
1522CT766.58.24
Applications
WB, IHC-P, FACS, IF
Purity
Protein G affinity
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the IRF3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This IRF3 antibody is available for research use only.
Storage Conditions
Aliquot the IRF3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the IRF3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, nuclear
Image Legend
Western blot testing of 1) monkey COS-7 and human 2) Daudi, 3) HepG2, 4) HT-29, 5) Jurkat and 6) MCF7 cell lysate with IRF3 antibody. Predicted molecular weight ~47 kDa.

Related Products

CatalogName
V4642-100UGInterferon regulatory factor 3 Antibody / IRF3
V4643-20UGInterferon regulatory factor 3 Antibody / IRF3
V4642SAF-100UGInterferon regulatory factor 3 Antibody / IRF3
RQ6374Interferon regulatory factor 3 Antibody / Irf3
V4642-20UGInterferon regulatory factor 3 Antibody / IRF3
V4643-100UGInterferon regulatory factor 3 Antibody / IRF3
RQ6384Interferon regulatory factor 3 Antibody / IRF3
V4643SAF-100UGInterferon regulatory factor 3 Antibody / IRF3
V5100SAF-100UGMITA Antibody / Mediator of IRF3 activation / STING1 / ERIS
V5100-100UGMITA Antibody / Mediator of IRF3 activation / STING1 / ERIS

Alternative Products

CatalogName
GTR18158643-01IRF3 Antibody

Popular Products