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ATP5O Antibody

CAT: 0800-F54457-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F54457-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25
UniProt
P48047
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 38-64 from the human protein was used as the immunogen for the ATP5O antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IF, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ATP5O antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ATP5O antibody is available for research use only.
Storage Conditions
Aliquot the ATP5O antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ATP5O antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human skeletal muscle tissue with ATP5O antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

UniProtKB · P48047

ATP synthase peripheral stalk subunit OSCP, mitochondrial

ATPO_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P48047
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ATP5PO
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B2R4E2, Q5U042, Q6IBI2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationUbl conjugation

Biological process

ATP synthesisHydrogen ion transportIon transportTransport

Disease

Disease variantPrimary mitochondrial disease

Cellular component

MembraneMitochondrionMitochondrion inner membrane

Domain

Transit peptide

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GTR17887941-01ATP5O Antibody

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