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PI4K2A Antibody

CAT: 0800-F54452-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F54452-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs. PI4K II is activated by detergent and inhibited by adenosine. Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P. Some cells overexpressing PI4KII have scattered or no perinuclear Golgi. Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi. RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network. PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35%. It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25
UniProt
Q9BTU6
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for the PI4K2A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PI4K2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PI4K2A antibody is available for research use only.
Storage Conditions
Aliquot the PI4K2A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the PI4K2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human kidney lysate with PI4K2A antibody. Predicted molecular weight ~54 kDa.

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