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EphA4 Antibody

CAT: 0800-F54444-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F54444-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families) .
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
UniProt
P54764
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 40-70 from the human protein was used as the immunogen for the EphA4 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC-P, FACS, WB
Purity
SAS precipitation
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the EphA4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This EphA4 antibody is available for research use only.
Storage Conditions
Aliquot the EphA4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the EphA4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human 1) HeLa and 2) NCI-H460 cell lysate with EphA4 antibody. Expected molecular weight: 110-130 kDa depending on glycosylation level.

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