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ZWINT Antibody

CAT: 0800-F54426-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F54426-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25
UniProt
O95229
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 59-88 from the human protein was used as the immunogen for the ZWINT antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IHC-P
Purity
SAS precipitation
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ZWINT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ZWINT antibody is available for research use only.
Storage Conditions
Aliquot the ZWINT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ZWINT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of mouse cerebellum lysate with ZWINT antibody. Predicted molecular weight ~31 kDa.

UniProtKB · O95229

Outer kinetochore KNL1 complex subunit ZWINT

ZWINT_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O95229
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ZWINT
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A6NNV6, Q0D2I3, Q9BWD0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCell divisionMitosis

Cellular component

CentromereChromosomeKinetochoreNucleus

Domain

Coiled coil

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