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UBE4B Antibody

CAT: 0800-F54394-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F54394-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4B represents an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. UBE4B also the strongest candidate among the neuroblastoma tumor suppressors.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25
UniProt
O95155
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 112-142 from the human protein was used as the immunogen for the UBE4B antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the UBE4B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This UBE4B antibody is available for research use only.
Storage Conditions
Aliquot the UBE4B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the UBE4B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear, cytoplasmic
Image Legend
Western blot testing of human 1) K562, 2) Raji, 3) HeLa and 4) NIH 3T3 lysate with UBE4B antibody. Predicted molecular weight ~146 kDa.

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