MAP1LC3B Antibody

• Description:

  The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

• Specifications:

  Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000

• UniProt:

  Q9GZQ8

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 9-33 from the human protein was used as the immunogen for the MAP1LC3B antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC-P, FACS, WB

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the MAP1LC3B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This MAP1LC3B antibody is available for research use only.

• Storage Conditions:

  Aliquot the MAP1LC3B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the MAP1LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC testing of FFPE human brain tissue with MAP1LC3B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.