ENT1 Antibody

CAT: 0800-F54342-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
CAT#:0800-F54342-0.4MLSize:0.4 mL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ENT1, or Equilibrative nucleoside transporter 1, is a member of the equilibrative nucleoside transporter family. It is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR) . Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25
UniProt
Q99808
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 402-431 from the human protein was used as the immunogen for the ENT1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ENT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ENT1 antibody is available for research use only.
Storage Conditions
Aliquot the ENT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ENT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human 1) brain, 2) breast and 3) placenta lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
Product Datasheet
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